VisiFibre

Our Solution

1. Sampling Process

1.1. COLLECTING SAMPLES

1.1 Collecting the Samples Setup

A filter is placed over the support connected to an air sampling pump, positioned on tables in the worker area. Process: The air pump absorbs air, leaving microplastics adhered to the filter. Transportation: Filters are carefully removed using a mobile tray and transported from the worker room to the control room.

1.2 Sampling Time and Volume

  • Duration: Aim for 1.5 hours of air aspiration.
  • Adjustments: 1 filter per 3 people, with the total time ranging between 1 to 2.5 hours.
  • Filter Specifications: Material – Polycarbonate coated with aluminum; Porosity – 5-6 µm.

1.3 Sample Collection Schedule

  • Weekly Schedule: Detailed daily time slots from Monday to Friday.
  • Room Capacity Considerations: Maximum of 3 Pipe 4.0 devices for larger rooms.

2. Pre-Raman Analysis Techniques

2.1 Weaknesses of Raman Analysis

  • Weak Raman signal and fluorescence interference due to impurities and additives.
  • Manual detection challenges using an optical microscope leading to possible false positives/negatives.

2.2 Presampling Solutions

  • Utilization of non-linear Raman techniques and cleaning protocols.
  • Photo-bleaching and algorithms to mitigate fluorescing agents.
  • Advanced image analysis software for particle detection.

3. Raman Spectroscopy Technologies

3.1 What is Raman Spectroscopy?

  • A non-destructive technique for chemical composition and molecular structure analysis.
  • Inelastic scattering of light (Raman effect) identifies molecules.

3.2 Basic Working Principles

  • Interaction: Incident light causes molecular vibrations.
  • Scattering: Changes in scattered light energy reveal molecular features.
  • Spectrum Analysis: X-Axis (Raman Shift), Y-Axis (Signal Intensity), Peaks (molecular vibrations).

3.3 Interpretation and Applications

  • Material and contaminant identification.
  • Analysis of polymers and plastics.
  • Research in nanotechnology and material sciences.

4. Pipe 4.0 Adaptation (microRaman for Solids)

4.1 Adjustments for Solid Samples

  • Modifications to accommodate the specific requirements for solid sample analysis.

5. Data Analysis

5.1 Database Results

  • Analysis of microplastic concentration per person and time of day/year.
  • Correlations with machine usage and environmental conditions.

5.2 Validating the Approach

  • Collaborations with CSIC Laboratory and experts in the field.
  • Review of scientific literature and interviews with researchers.